Sample Size and Experimental Design for Methane Experiments in Dairy Cows

Select methane measurement technique: Choose from open-circuit respirometry chambers, the GreenFeed system, or the SF6 technique.
Select experimental design type: Choose from Latin Square, Cross-Over, Randomized Complete Block, or Repeated Measures Designs.
Define the experimental setup: Enter the number of treatments (different conditions or variables) and periods (for multiple measurement designs).
Set expected CH4 yield reduction: Adjust the expected CH4 yield and its variability if needed.
Define calculation parameters: Use default values (alpha = 0.05, power = 0.95, correlation = 0.5) or adjust them.
Calculate sample size: Click the "RUN" button to calculate.
Review results: Check the total sample size and the descriptive table of the experimental design.
Adjust and recalculate: Modify inputs and recalculate if needed.

The values for methane yield and pooled standard deviation (SD) are based on studies conducted in Canada, the USA, and Europe from 2012 to 2023.

Sample Size Calculator

The CH4 yield and its variability (SD) are average values from the database. You can customize these values.

Methane Technique Experimental Design Methane Yield (g CH4/kg DMI) Alpha (Type I Error Rate, α)
Number of Treatments Expected CH4 Yield Reduction (%) Power (1 - β)
Number of Periods or Blocks Pooled SD of CH4 Yield Correlation (ρ) Total Sample Size